The player sneaks around in the dark at a cow ranch, and searches for the calf to kill and feed to his/her pups. In the game, players have the opportunity (though not a mandatory option) to raid a cattle ranch for extra food in the Night Mission. Wolf territories will also expand, so the player cannot take an easy way out. The only way pups can get across the river is by finding a shallow sandbar. But the player, the player's mate, and pups will run into grizzlies, coyotes, eagles and drowning. At the end, you, your mate, and your surviving pups must make a journey for the summer den in the Douglas fir forests. Ravenous bears, coyotes, eagles, cougars, and wolves roam the earth, posing a new threat to young pups as they wait to snatch one up under a novice parent's nose. After completion, the player will obtain pups. However, after finding a densite, the player must scent mark the area around the den to make it safe from predators and stranger wolves. Upon leaving Amethyst Mountain, players (with their mate) enter the Slough Creek map and search for a den to raise pups in a new location, Slough Creek. Just like real wolves, males are larger in size than females. WolfQuest 2.7 added many new customization features, including more pelts as well as injuries and a radio collar. There are a variety of pelts to choose from (organized by packs), with two sliders that let players change the tints of the guard hairs and undercoat of the wolf.
When creating their wolf character, players are free to choose the gender, fur coloration, stats, and name of their wolf. In order to find a mate, the player must first earn 800 experience points, mainly by hunting elk, and then search for a mate which can be found in all territories. They have the option to find a mate, which, when successful, will follow and aid the player throughout the rest of the game.
However, it is possible to fight off the bears and coyotes by chasing them, and fight off other wolves. They must also avoid dangers such as grizzly bears, coyotes, and non-dispersal wolves.
To do so they must hunt elk, moose, and hares, or feed off elk carcasses. In single player, the player must survive as a dispersal gray wolf in Yellowstone National Park. WolfQuest was initially developed episodically, and these episodes now form the main single-player game arc: Gameplay is divided into two parts – single player, where players are able to play in a "story mode" type adventure, and multiplayer, where players are able to play in real-time with up to 7 other people in a player-created game server.
While the old, free version of the game remains available, development efforts are now focused on new versions, particularly WolfQuest 3: Anniversary Edition, a complete remake and expansion of the game. In November 2015, Eduweb released a WolfQuest 2.7, an improved and expanded version of the game for purchase.
The game was originally funded by grants from the National Science Foundation, Best Buy, and other foundations and donors and distributed as a free download for Mac and Windows computer. WolfQuest challenges players to learn about wolf ecology by living the life of a wild wolf in Yellowstone National Park and is a family-friendly, fun game for people of all ages. The game's main purpose is to help players understand wolves and the roles they play in nature by being virtually incarnated as a gray wolf themselves. WolfQuest is a 3D wildlife simulation video game originally developed by the Minnesota Zoo and game developer company Eduweb, and developed solely by Eduweb since 2013.